Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells.
Identifieur interne : 000150 ( Main/Exploration ); précédent : 000149; suivant : 000151Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells.
Auteurs : Donatella D'Eliseo [Italie] ; Giuliana Di Rocco [Italie] ; Rossella Loria [Italie] ; Silvia Soddu [Italie] ; Angela Santoni [Italie] ; Francesca Velotti [Italie]Source :
- Journal of experimental & clinical cancer research : CR [ 1756-9966 ] ; 2016.
Abstract
Granzyme B (GrB) is a serine protease, traditionally known as expressed by cytotoxic lymphocytes to induce target cell apoptosis. However, it is emerging that GrB, being also produced by a variety of normal and neoplastic cells and potentially acting on multiple targets, might represent a powerful regulator of a wide range of fundamental biological processes. We have previously shown that GrB is expressed in urothelial carcinoma tissues and its expression is associated to both pathological tumor spreading and EMT. We have also shown that docosahexaenoic acid (DHA), a dietary ω-3 polyunsaturated fatty acid with anti-tumor activity, while inhibiting urothelial and pancreatic carcinoma cell invasion also inhibited their GrB expression in vitro. In this study, we characterized a panel of colorectal carcinoma (CRC) cells, with different invasive capabilities, for GrB expression and for the contribution of GrB to their EMT and invasive phenotype. In addition, we investigated the effect of DHA on CRC cell-associated GrB expression, EMT and invasion.
DOI: 10.1186/s13046-016-0302-6
PubMed: 26830472
Affiliations:
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cell apoptosis. However, it is emerging that GrB, being also produced by a variety of normal and neoplastic cells and potentially acting on multiple targets, might represent a powerful regulator of a wide range of fundamental biological processes. We have previously shown that GrB is expressed in urothelial carcinoma tissues and its expression is associated to both pathological tumor spreading and EMT. We have also shown that docosahexaenoic acid (DHA), a dietary ω-3 polyunsaturated fatty acid with anti-tumor activity, while inhibiting urothelial and pancreatic carcinoma cell invasion also inhibited their GrB expression in vitro. In this study, we characterized a panel of colorectal carcinoma (CRC) cells, with different invasive capabilities, for GrB expression and for the contribution of GrB to their EMT and invasive phenotype. In addition, we investigated the effect of DHA on CRC cell-associated GrB expression, EMT and invasion.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Latium</li></region><settlement><li>Rome</li></settlement></list><tree><country name="Italie"><region name="Latium"><name sortKey="D Eliseo, Donatella" sort="D Eliseo, Donatella" uniqKey="D Eliseo D" first="Donatella" last="D'Eliseo">Donatella D'Eliseo</name></region><name sortKey="Di Rocco, Giuliana" sort="Di Rocco, Giuliana" uniqKey="Di Rocco G" first="Giuliana" last="Di Rocco">Giuliana Di Rocco</name><name sortKey="Loria, Rossella" sort="Loria, Rossella" uniqKey="Loria R" first="Rossella" last="Loria">Rossella Loria</name><name sortKey="Santoni, Angela" sort="Santoni, Angela" uniqKey="Santoni A" first="Angela" last="Santoni">Angela Santoni</name><name sortKey="Soddu, Silvia" sort="Soddu, Silvia" uniqKey="Soddu S" first="Silvia" last="Soddu">Silvia Soddu</name><name sortKey="Velotti, Francesca" sort="Velotti, Francesca" uniqKey="Velotti F" first="Francesca" last="Velotti">Francesca Velotti</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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